探索猪蓝耳病(PRRS)防控与净化的两例分析报告

  猪繁殖与呼吸障碍综合症(PRRS)以怀孕母猪流产和各日龄仔猪呼吸系统疾病为特征。在中国,猪繁殖与呼吸障碍综合症病毒(PRRSV)通过突变和重组已经迅速发生变异,导致出现不同致病力和毒力的毒株。

  PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME (PRRS) IS CHARACTERIZED BY REPRODUCTIVE LOSSES IN PREGNANT SOWS AND RESPIRATORY DISEASE IN PIGS OF ALL AGES. PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS (PRRSV) IN CHINA HAS RAPIDLY CHANGED THROUGH MUTATION AND RECOMBINATION, RESULTING IN MANY ISOLATES WITH DIFFERENT LEVELS OF PATHOGENICITY AND VIRULENCE.

  本研究探讨了在中国一个现代化农场和一个传统农场建立并实行的PRRSV的防控和净化方法。

  HERE IS REPORTED A METHOD FOR CONTROL AND ERADICATION OF PRRSV WHICH WAS DEVELOPED AND IMPLEMENTED ON A MODERN-STYLE AND A TRADITIONAL-STYLE CHINESE FARM.

  材料和方法

  MATERIALS AND METHODS

  在初步诊断农场暴发PRRS后,检查并改进了生物安全措施。引进足够农场4个月需要的后备母猪。

  AFTER THE INITIAL DIAGNOSIS OF PRRS OUTBREAK ON THE FARM, BIOSECURITY ROUTINES ARE REVIEWED AND IMPROVED. SUFFICIENT GILTS ARE INTRODUCED TO SUPPLY THE DEMAND FOR A 4 MONTH PERIOD.

  然后免疫猪群灭活苗并停止引种。等猪群免疫力稳定后,断奶产生蓝耳阴性仔猪,然后执行部分清群来净化蓝耳。

  THE HERD IS THEN VACCINATED WITH INACTIVATED PRRS VACCINE AND CLOSED TO ALL ANIMAL INPUTS. AFTER STABILIZING IMMUNITY IS ACHIEVED, PRRS NEGATIVE PIGLETS CAN BE WEANED, AND PARTIAL DEPOPULATION IS PERFORMED TO ERADICATE PRRSV.

  结果

  RESULTS

  通过现场检查,病理学,PCR和测序诊断出一个现代化一点式农场(M农场)和一个传统一点式农场(T农场)分别感染NADC30类PRRS毒株和JXA1类PRRS毒株(图1和2)

  A MODERN-STYLE ONE-SITE OPERATION (M FARM) AND TRADITIONAL-STYLE ONE-SITE OPERATION (T FARM) WERE DIAGNOSED RESPECTIVELY WITH NADC30-LIKE AND JXA1-LIKE PRRS STRAINS BY CLINICAL EXAMINATION, PATHOLOGY, PCR AND SEQUENCE ANALYSIS (FIGURES 1 AND 2).

  现场调查发现产房和保育大部分仔猪呼吸窘迫,咳嗽,打喷嚏,有结膜炎,发烧,体温高达41.4度。

  WEAK, SEVERELY DEPRESSED PIGS WERE NOTED IN MOST LITTERS. MANY PIGS HAD RESPIRATORY DISTRESS AND CONJUNCTIVITIS. BODY TEMP WAS NEAR NORMAL IN MANY PIGS BUT SOME SHOWED FEVER UP TO 41.4°C.

  发病前后窝均健仔从9.24降到6.33和窝断奶合格仔从9.23降到4.87。母猪流产。

  HEALTHY BORN DROPPED FROM 9.24 TO 6.33 AND AVERAGE WEANER PER LITTER DECREASED FROM 9.23 TO 4.87. ABORTION.

  

  解剖发现产房仔猪有中度间质性肺炎。

  NECROPSY FINDING SHOWED THE PIGLETS HAD MILD INTERSTITIAL PNEUMONIA.

  

  PCR检测为蓝耳阳性。

  PCR PRRS POSITIVE

  

  测序结果

  SEQUENCEING

 

  间质性肺炎

  INTERSTITIAL PNEUMONIA.

  

  生物安全检查发现,卡车清洗消毒不严格。

  BIOSECURITY AUDIT FOUND UNCLEANED TRUCK FOR TRANSPORTATION

  执行防控措施后40天,母猪群PRRS抗原阴性,母猪群横向传播停止。

  TEST DATA INDICATETHAT THE PRRS VIRUS IS NOT BEING TRANSMITTED HORIZONTALLY IN THE SOW HERD FROMSOW TO SOW

 

  母猪大部分PRRS抗体阳性。

  MOST SOWS ARE PRRS ANTIBODY POSITIVE

  

  产房各个日龄仔猪均维持PRRS抗原阴性,母猪向仔猪的垂直传播停止。

  TESTDATA INDICATE THAT THE PRRS VIRUS IS NOT BEING TRANSMITTED VERTICALLY FROM SOWTO PIG

  

  Two-wayANOVA 分析,母猪体内抗体水平和其哺乳的仔猪体内的抗体水平差异不显著,提示仔猪体内抗体来自于母乳。

  THEANTIBODY LEVELS OF SOWS AND THEIR OFFSPRING ARE NOT SIGNIFICANT DIFFERENTINDICATED THE PIGLETS ANTIBODY IS MATERNAL ANTIBODY.

  

  在蓝耳净化措施开始执行后的40天内,母猪群和断奶仔猪变为PRRS 抗原阴性,并在接下来的3次月度检测或者半月检测中依然保持阴性。

  WITHIN 40 DAYS OF THE INITIATION OF PRRS ELIMINATION MEASURES, THE SOW HERD AND WEANLING PIGLETS BECAME PCR NEGATIVE FOR PRRS AND REMAINED NEGATIVE IN THE NEXT 3 MONTHLY OR BI-WEEKLY TESTS.

  然后,所有的保育猪和育肥猪被转移走,并彻底清洗和消毒保育舍和育肥舍。

  ALL THE WEANERS AND GROWER/FINISHER PIGS WERE REMOVED FROM THE FARM AND ALL BUILDINGS WERE CLEANED AND DISINFECTED.

  在M农场,保育舍空置14天后,断奶仔猪被转移到保育舍饲养。

  IN THE M FARM, AFTER THE NURSERY BARNS WERE VACANT FOR 14 DAYS, THE WEANLING PIGLETS WERE TRANSFERRED INTO NURSERY BARNS AND THRIVED.

  案例2

  CASE 2

  从蓝耳暴发前1个月到爆发后5个月共7个月的部分生产指标分析

  PARTIAL PRODUCTION RESULTS FROM 1 MONTH BEFORE TO 5 MONTH AFTER OUTBREAK.

  

  结果

  RESULTS

  发病前后窝均总仔从13.38降到12.04,窝均健仔从12.61降到11.06,窝断奶合格仔从10.82降到10.13。

  TOTAL BORN DROPPED FROM13.38 TO 12.04 WHILE HEALTHY BORN AND WEANERS PER LITTER DECREASED FROM 12.61 AND 10.82 TO 11.06 AND10.13
 

  现场调查发现产房部分仔猪呼吸窘迫,咳嗽,打喷嚏,有结膜炎,发烧,体温高达41.5度。

  WEAK, SEVERELY DEPRESSED PIGS WERE NOTED IN MOST LITTERS. MANY PIGS HAD RESPIRATORY DISTRESS AND CONJUNCTIVITIS. BODY TEMP WAS NEAR NORMAL IN MANY PIGS BUT SOME SHOWED FEVER UP TO 41.5°C.

  解剖发现产房仔猪有中度间质性肺炎。

  NECROPSY FINDING SHOWED THE PIGLETS HAD MILD INTERSTITIAL PNEUMONIA.

  

  PCR检测为蓝耳阳性。

  PCR RESULT FOUND PRRS IS POSITIVE

  

  测序结果

  SEQUENCING RESULTS

 

  间质性肺炎

  INTERSTITIAL PNEUMONIA

  

  生物安全检查发现,卡车清洗消毒不严格。

  BIOSECURITY AUDIT FOUND UNCLEANED TRUCK FOR TRANSPORTATION

  执行防控措施后40天,母猪群PRRS抗原阴性,母猪群横向传播停止。

  TEST DATA INDICATE THAT THE PRRS VIRUSIS NOT BEING TRANSMITTED HORIZONTALLY IN THE SOW HERD FROM SOW TO SOW

  

  产房各个日龄仔猪均维持PRRS抗原阴性,母猪向仔猪的垂直传播停止。

  TEST DATA INDICATETHAT THE PRRS VIRUS IS NOT BEING TRANSMITTED VERTICALLY FROM SOW TO PIG

  保育阶段仔猪为PRRS抗原阳性,表明PRRS病毒在保育阶段存在横向传播。

  TEST DATA INDICATE THAT THE PRRS VIRUS IS BEING TRANSMITTED HORIZONTALLY IN NURSERY

  在T农场,断奶猪被转移到一个租来的设备十分简陋的农场。这些感染蓝耳的断奶猪出现了很高的死亡率,因而部分清群计划被迫中止。

  IN THE T FARM, THE WEANERS WERE REMOVED TO A RENTED FARM WITH VERY BASIC FACILITIES. THERE WAS HIGH MORTALITY AMONG THOSE PRRS INFECTED PIGLETS AND THE PARTIAL DEPOPULATION PLAN WAS INTERRUPTED.

  从蓝耳暴发前1个月到爆发后4个月共6个月的部分生产指标分析

  PARTIAL PRODUCTION RESULTS OF 6 MONTHS FROM 1 MONTH BEFORE TO 4 MONTH AFTER OUTBREAK.

  

  讨论

  DISCUSSION

  本研究讨论的方法在40天内产生PRRS阴性断奶猪,繁殖性能在2个月内提升到PRRS爆发之前的水平。

  THE METHOD DISCUSSED HERE PRODUCED PRRS NEGATIVE WEANERS IN 40 DAYS AND REPRODUCTION WAS IMPROVED TO THE SAME LEVEL BEFORE PRRS OUTBREAK WITHIN 2 MONTHS.

  该方法在PRRS净化中发挥了至关重要的作用,并大幅度降低了经济损失。在未来,多点式农场会是彻底净化蓝耳的一个方案。

  THIS METHOD CAN PLAY A KEY ROLE IN PRRS ELIMINATION AND REDUCE THE ECONOMIC LOSSES DRAMATICALLY. MULTI-SITE OPERATION MAY BE A SOLUTION TO COMPLETELY ERADICATE PRRS IN FUTURE.

[责任编辑:猪小妹 ]
标签

PRRS

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